Taurusinc

TAURUSDISPO® Taq 2x PCR Master Mix with Dye

TAURUSDISPO® Taq DNA Polymerase possesses a 5′–→3′ Polymerase activity and a 3′ end of added adenine (A) activity.

Taq 2x master mix with dye is an optimized, ready to use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCL and stabilizers, as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4kb and Tartrazine migrates at ~10kb. Both dye are present in concentrations that do not mask comigrating DNA bands.

It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products.

It can amplify upto 4kb from complex genomic DNA or upto 5kb from λDNA.

Applicable to the PCR reaction, colony PCR and Primer Extension.

 

 

Cat # Product Description HSN Code Price
TI-MB-20604S Taq 2x PCR Master Mix with Dye, 100 rxn 38220019 ₹ 4,000.00
TI-MB-20604L Taq 2x PCR Master Mix with Dye, 500 rxn 38220019 ₹ 16,000.00

Description

TAURUSDISPO® Taq DNA Polymerase possesses a 5′–→3′ Polymerase activity and a 3′ end of added adenine (A) activity.

Taq 2x master mix with dye is an optimized, ready to use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCL and stabilizers, as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4kb and Tartrazine migrates at ~10kb. Both dye are present in concentrations that do not mask comigrating DNA bands.

It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products.

It can amplify upto 4kb from complex genomic DNA or upto 5kb from λDNA.

Applicable to the PCR reaction, colony PCR and Primer Extension.

Product spec

5′–→3′ Exonuclease : YES
3′–→5′ Exonuclease : No
Heat Inactivation: No
Strand Displacement : +
Resulting Ends: Single base 3′ Overhangs
Storage Temp: -200C

Master Mix Composition:
10mM Tris HCL, 1.5mM MgCl2, 50mM KCL, 0.08% IPGAL 630, 0.05% Tween 20, pH 8.6@250C, 200µM dNTP’s, 5% Glycerol, 25U/ml Taq DNA polymerase, 1X Xylene Cyanol, 1X Tartrazine.

Data Sheets



taurusdispo-taq-2x-pcr-master-mix-with-dye