The TAURUSDISPO® Whole Blood RNA Mini Kit is designed for rapid and efficient isolation of total RNA from frozen and fresh whole blood samples. The kit uses a chaotropic saltbased formula for cell lysis, RNase inactivation and RNA binding (>200 nt, e.g., 18S, 28S RNA, pri-miRNA) to silica membrane. The optional on-column DNase I digestion technique offers a convenient DNA removal method.
The high-purity RNA is eluted from the membrane by using a low-ionic-strength buffer.
This extracted total RNA is ready-to-use for downstream applications, such as real-time RT-PCR, cDNA synthesis, Northern blotting, primer extension, miRNA selection, etc.
Format/Principle: Mini spin column (silica matrix)
Sample Size: 200~400 µl whole blood
Size of Isolated RNA: >200 nucleotides
Typical RNA Yield: 5~7 µg/ 1 ml whole blood
Operation Time: ≤50 minutes
Binding Capacity: Up to 100 µg/RNA binding column
Column Applicability: Centrifugation and vacuum
Minimum Elution Volume: 20 µl/RNA binding column